#

KODE KURSUS

INBIO 23

KURSUS DIMULAI

NOV 2020

KURSUS BERAKHIR

NOV 2020

ESTIMASI WAKTU BELAJAR

2 JAM/MINGGU

HARGA: Rp. 250.000,-



Tentang Kursus:



Pada topik ini akan dibahas mengenai konsep mengenai ekpresi protein rekombinan dan aplikasinya serta langkah-langkah dalam purifikasi protein.




Topik Kursus



MINGGU 1
pukul
Konsep Protein
• Why do we express recombinant protein?
• Aplikasinya apa saja?
• Guideline step by step purifikasi protein secara umum dari tahap isolasi hingga analisis
• Persiapan dan istilah yang harus dipelajari pada term selanjutnya

MINGGU 2
pukul
Isolation and Cloning
• Protein expression host of choise based on Pubmed
• Workflow from gene to protein How to choose expression vector
• Insert target gene – PCR workflow
• Transfer cloned DNA to the host strain.
• Express the protein (in E. coli)
• Evaluate protein yield with SDS, immunoblotting, 2D-Page detection.

MINGGU 3
pukul
Cell culture
• Inkubasi hingga OD tertentu
• Induce IPTG
• Hal yang perlu diperhatikan: Temperatur, pH, Antibiotik

MINGGU 4
pukul
Sample preparation
• Cell harvest
• Intracellular protein; lisis, sonikasi, homogenisasi
• Ekstrasellular protein

MINGGU 5
pukul
Protein Purification
• Affinity resin, affinity chromatography
• Size exclusion chromatography
• His-tag column
• Protein purification system
• Detection on SDS -Page

MINGGU 6
pukul
Protein Analysis
• Evaluate protein yield; SDS -Page, 2D -Page, Western blot
• Identify fraction / separate protein by size using protein purifier system