This introductory online course explores the fundamentals and advanced applications of digital PCR (dPCR) in biomedical research. Participants will begin by reviewing core PCR technologies before delving into the principles that set digital PCR apart, including its partitioning strategies and enhanced quantification capabilities. Through real-world examples, learners will discover how dPCR is revolutionizing diagnostics, gene expression studies, and rare mutation detection. The course also covers best practices for experimental design to ensure reliable and reproducible results. A hands-on simulation will guide participants through digital PCR data analysis, interpretation, and contextualization within biomedical research, making this course ideal for life science researchers, graduate students, and clinicians seeking to integrate dPCR into their research toolkit.

• Fundamentals of PCR Technologies
• Principles of Digital PCR 
• Applications in Biomedical Research
• Experimental Design for Digital PCR
• Hands on: Data Analysis Simulation and Interpreting Results in a Biomedical Context